MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice

摘要: Plant height and tillering are crucial factors determining rice plant architecture influencing grain production. In this study, multi-tillering dwarf1 (mtd1), a stable multi-tiller dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica variety Wuyunging7. Compared with wild type, mtd1 mutant exhibited pleiotropic phenotypes, including dwarfism, more tillers, brittle culms delayed heading date. By employing map-based cloning strategy, gene MTD1 finally mapped to an approximately 66-kb region on short arm of chromosome 9. Sequencing results showed that LOC_Os09g02650 (BC12) in had single nucleotide substitution (G A), which generated premature translation stop. Over-expressing MTD1/BC12 coding sequence rescued all phenotypes mutants confirms BC12 is mutated mutant. Quantitative reverse transcription-PCR analysis could negatively regulate expression MONOCULM 1, IDEAL PLANT ARCHITECTURE1 Tillering Dwarf control tillering. Remarkably, α-amylase activity gibberellic acid (GA) treatment phenotype dependent GA biosynthesis pathway. These facilitated further uncover molecular mechanism growth development rice.