Applications of Digital PCR for Clinical Microbiology.
作者： Jane Kuypers , Keith R. Jerome
DOI: 10.1128/JCM.00211-17
关键词: Standard curve 、 Real-time polymerase chain reaction 、 Microbiology 、 Absolute quantification 、 Computer science 、 Clinical microbiology 、 Digital polymerase chain reaction 、 Computational biology
摘要: … Assays that require high precision for measurement of viral load, testing of samples that contain inhibitors of PCR amplification, amplification of targets by consensus primer and probe …
sci-hub.se 参考文章(43)
Gerwyn M. Jones, Eloise Busby, Jeremy A. Garson, Paul R. Grant, Eleni Nastouli, Alison S. Devonshire, Alexandra S. Whale, Digital PCR dynamic range is approaching that of real-time quantitative PCR Biomolecular Detection and Quantification. ,vol. 10, pp. 31- 33 ,(2016) , 10.1016/J.BDQ.2016.10.001
Ruth Hall Sedlak, Thuy Nguyen, Isabel Palileo, Keith R. Jerome, Jane Kuypers, Superiority of Digital Reverse Transcription-PCR (RT-PCR) over Real-Time RT-PCR for Quantitation of Highly Divergent Human Rhinoviruses. Journal of Clinical Microbiology. ,vol. 55, pp. 442- 449 ,(2017) , 10.1128/JCM.01970-16
Huiyu Low, Shun-Jie Chan, Guo-Hao Soo, Belinda Ling, Eng-Lee Tan, Clarity™ digital PCR system: a novel platform for absolute quantification of nucleic acids Analytical and Bioanalytical Chemistry. ,vol. 409, pp. 1869- 1875 ,(2017) , 10.1007/S00216-016-0131-7
Cristian Koepfli, Wang Nguitragool, Natalie E. Hofmann, Leanne J. Robinson, Maria Ome-Kaius, Jetsumon Sattabongkot, Ingrid Felger, Ivo Mueller, Sensitive and accurate quantification of human malaria parasites using droplet digital PCR (ddPCR). Scientific Reports. ,vol. 6, pp. 39183- ,(2016) , 10.1038/SREP39183
Alexander L. Greninger, Allen C. Bateman, Ederlyn E. Atienza, Sharon Wendt, Negar Makhsous, Keith R. Jerome, Linda Cook, Copy Number Heterogeneity of JC Virus Standards. Journal of Clinical Microbiology. ,vol. 55, pp. 824- 831 ,(2017) , 10.1128/JCM.02337-16
Allen C Bateman, Alexander L Greninger, Ederlyn E Atienza, Ajit P Limaye, Keith R Jerome, Linda Cook, Quantification of BK Virus Standards by Quantitative Real-Time PCR and Droplet Digital PCR Is Confounded by Multiple Virus Populations in the WHO BKV International Standard. Clinical Chemistry. ,vol. 63, pp. 761- 769 ,(2017) , 10.1373/CLINCHEM.2016.265512
Emmanuelle Jeannot, Véronique Becette, Maura Campitelli, Marie‐Ange Calméjane, Emmanuelle Lappartient, Evelyne Ruff, Stéphanie Saada, Allyson Holmes, Dominique Bellet, Xavier Sastre‐Garau, Circulating human papillomavirus DNA detected using droplet digital PCR in the serum of patients diagnosed with early stage human papillomavirus-associated invasive carcinoma. The Journal of Pathology: Clinical Research. ,vol. 2, pp. 201- 209 ,(2016) , 10.1002/CJP2.47
John M. S. Bartlett, David Stirling, A short history of the polymerase chain reaction. Methods of Molecular Biology. ,vol. 226, pp. 3- 6 ,(2003) , 10.1385/1-59259-384-4:3
Jernej Pavšič, Jana Žel, Mojca Milavec, Digital PCR for direct quantification of viruses without DNA extraction Analytical and Bioanalytical Chemistry. ,vol. 408, pp. 67- 75 ,(2016) , 10.1007/S00216-015-9109-0
Feng Shen, Bing Sun, Jason E. Kreutz, Elena K. Davydova, Wenbin Du, Poluru L. Reddy, Loren J. Joseph, Rustem F. Ismagilov, Multiplexed quantification of nucleic acids with large dynamic range using multivolume digital RT-PCR on a rotational SlipChip tested with HIV and Hepatitis C viral load Journal of the American Chemical Society. ,vol. 133, pp. 17705- 17712 ,(2011) , 10.1021/JA2060116