Global impact of diet and temperature over aquaculture of Octopus vulgaris paralarvae from a transcriptomic approach.

摘要: Common octopus, Octopus vulgaris, is an economically important cephalopod species. However, its rearing under captivity currently challenged by massive mortalities previous to their juvenile stage due nutritional and environmental factors. Dissecting the genetic basis regulatory mechanism behind this mortality requires genomic background knowledge. A transcriptomic sequencing of 10 dph octopus paralarvae from different experimental conditions was constructed via RNA-seq. total 613,767,530 raw reads were filtered de novo assembled into 363,527 contigs which 82,513 annotated in UniProt carrying also GO KEGG information. Differential gene expression analysis carried out on reared diet regimes temperatures, including wild paralarvae. Genes related lipid metabolism exhibited higher transcriptional levels individuals whose includes crustacean zoeas, had impact over development immune response capability. High temperature induces acclimation processes at time that increase metabolic demands oxidative stress. Wild show profile unexpectedly similar Artemia fed individuals. Proteomic results support hypothesis revealed analysis. The comparative study O. vulgaris profiles allowed identification genes deserve be further studied as candidates for biomarkers health. obtained here variations caused will provide new perspectives understanding molecular mechanisms requirements common open opportunities deepen requirements.