The Repolarizing Effects of Volatile Anesthetics on Porcine Tracheal and Bronchial Smooth Muscle Cells
作者： Michiaki Yamakage , Xiangdong Chen , Akira Kimura , Sohshi Iwasaki , Akiyoshi Namiki
DOI: 10.1097/00000539-200201000-00016
关键词: Pharmacology 、 Medicine 、 Bronchodilatation 、 Carbachol 、 Anesthetic 、 Channel blocker 、 Anesthesia 、 Sevoflurane 、 Muscle contraction 、 Muscarinic agonist 、 Muscarinic acetylcholine receptor
摘要: UNLABELLED This study was conducted to determine the effects of volatile anesthetics (potent bronchodilators) on membrane potentials in porcine tracheal and bronchial smooth muscle cells. We used a current-clamp technique examine isoflurane (1.5 minimum alveolar anesthetic concentration [MAC]) sevoflurane MAC) (third- fifth-generation) cells depolarized by muscarinic agonist, carbachol (1 microM). The receptor binding affinity were also investigated using radiolabeled assay technique. induced significant repolarization cell membranes trachea (from -19.8 -23.6 mV -24.8 mV, respectively) bronchus -24.7 -29.3 -30.4 without affecting receptor. repolarizing effect abolished Ca(2+)-activated Cl(-) channel blocker, niflumic acid. These results indicate that anesthetic-induced airway might be caused change activity different repolarized could part contribute contractions. IMPLICATIONS By use technique, agonist. mainly activity, not K(+) activity.
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