Characterization of the Dual Subcellular Localization of Lilium LsGRP1, a Plant Class II Glycine-Rich Protein
作者： Chia-Hua Lin , Chao-Ying Chen
DOI: 10.1094/PHYTO-01-14-0020-R
关键词: Subcellular localization 、 Protein purification 、 Lilium 、 Green fluorescent protein 、 Biology 、 Salicylic acid 、 Biochemistry 、 Cell wall 、 Phloem 、 Regulation of gene expression
摘要: The defense-related gene LsGRP1 exhibits an increased level of expression in Lilium spp. after being infected by Botrytis elliptica, the fungal pathogen lily leaf blight. In this study, profile protein (a plant class II glycine-rich protein) was characterized biochemically and its subcellular localization leaves evaluated using immunohistochemistry, enhanced green fluorescent (EGFP) imaging, extraction analysis. Using LsGRP1-specific antibody, found to be most abundant epidermal cells phloem tissues. Leaves from plants at different growth stages demonstrated similar levels 14- 16-kDa a decreased amount 23-kDa senescence stage. LsGRP1-EGFP imaging assays revealed that 14-kDa located plasma membrane whereas 16- weakly bound cell wall. time course analyses response salicylic acid treatment or B. elliptica infection showed accumulation over time. Because could detected ubiquitin conversion via mono-ubiquitination presumed, which is phenomenon has not been reported for protein.
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sci-hub.se 参考文章(40)
Chung JuiChou, Lu YuYen, S. N. Acharya, Chen ChaoYing, H. C. Huang, Induced host resistance against Botrytis leaf blight. Advances in plant disease management. pp. 259- 267 ,(2003)
Linda Hicke, Protein regulation by monoubiquitin Nature Reviews Molecular Cell Biology. ,vol. 2, pp. 195- 201 ,(2001) , 10.1038/35056583
R.M. BOSTOCK, Signal conflicts and synergies in induced resistance to multiple attackers Physiological and Molecular Plant Pathology. ,vol. 55, pp. 99- 109 ,(1999) , 10.1006/PMPP.1999.0218
Carolyn M. Ott, Vishwanath R. Lingappa, Integral membrane protein biosynthesis: why topology is hard to predict. Journal of Cell Science. ,vol. 115, pp. 2003- 2009 ,(2002) , 10.1242/JCS.115.10.2003
A. Martiniere, I. Lavagi, G. Nageswaran, D. J. Rolfe, L. Maneta-Peyret, D.-T. Luu, S. W. Botchway, S. E. D. Webb, S. Mongrand, C. Maurel, M. L. Martin-Fernandez, J. Kleine-Vehn, J. Friml, P. Moreau, J. Runions, Cell wall constrains lateral diffusion of plant plasma-membrane proteins Proceedings of the National Academy of Sciences of the United States of America. ,vol. 109, pp. 12805- 12810 ,(2012) , 10.1073/PNAS.1202040109
Damian D. Guerra, Judy Callis, Ubiquitin on the Move: The Ubiquitin Modification System Plays Diverse Roles in the Regulation of Endoplasmic Reticulum- and Plasma Membrane-Localized Proteins Plant Physiology. ,vol. 160, pp. 56- 64 ,(2012) , 10.1104/PP.112.199869
Daniel Marino, Nemo Peeters, Susana Rivas, Ubiquitination during Plant Immune Signaling Plant Physiology. ,vol. 160, pp. 15- 27 ,(2012) , 10.1104/PP.112.199281
Gilberto Sachetto-Martins, Luciana O Franco, Dulce E de Oliveira, Plant glycine-rich proteins: a family or just proteins with a common motif? Biochimica et Biophysica Acta. ,vol. 1492, pp. 1- 14 ,(2000) , 10.1016/S0167-4781(00)00064-6
Chia-Hua Lin, Min-Wei Chang, Chao-Ying Chen, A Potent Antimicrobial Peptide Derived from the Protein LsGRP1 of Lilium Phytopathology®. ,vol. 104, pp. 340- 346 ,(2014) , 10.1094/PHYTO-09-13-0252-R
U. Haupts, S. Maiti, P. Schwille, W. W. Webb, Dynamics of fluorescence fluctuations in green fluorescent protein observed by fluorescence correlation spectroscopy Proceedings of the National Academy of Sciences of the United States of America. ,vol. 95, pp. 13573- 13578 ,(1998) , 10.1073/PNAS.95.23.13573