Interaction of Human DNA Polymerase η with Monoubiquitinated PCNA: A Possible Mechanism for the Polymerase Switch in Response to DNA Damage
作者： Patricia L Kannouche , Jonathan Wing , Alan R Lehmann
DOI: 10.1016/S1097-2765(04)00259-X
关键词: DNA replication 、 DNA polymerase II 、 DNA polymerase 、 DNA polymerase delta 、 REV1 、 DNA clamp 、 Biology 、 DNA polymerase eta 、 Molecular biology 、 Primase
摘要: Most types of DNA damage block replication fork progression during synthesis because replicative polymerases are unable to accommodate altered bases in their active sites. To overcome this block, eukaryotic cells employ specialized translesion (TLS) polymerases, which can insert nucleotides opposite damaged bases. In particular, TLS by polymerase eta (poleta) is the major pathway for bypassing UV photoproducts. How cell switches from at site blocked forks unknown. We show that, human cells, PCNA becomes monoubiquitinated following irradiation and that dependent on hRad18 protein. Monoubiquitinated but not unmodified specifically interacts with poleta, we have identified two motifs poleta involved interaction. Our findings provide an attractive mechanism monoubiquitination might mediate switch.
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