Identification of a possible nucleotide binding site in CheW, a protein required for sensory transduction in bacterial chemotaxis.
作者： A Stock , J Mottonen , T Chen , J Stock
DOI: 10.1016/S0021-9258(19)75814-1
关键词: Biology 、 Gene 、 Binding site 、 Peptide sequence 、 Nucleic acid sequence 、 Biochemistry 、 Chemotaxis 、 Molecular biology 、 Nucleotide 、 Gel electrophoresis 、 Escherichia coli 、 Cell biology
摘要: CheW is an essential component of the system which mediates chemotaxis in Salmonella typhimurium and Escherichia coli. Here we report nucleotide sequence cheW gene as well purification characterization protein. The DNA predicts a protein 18,000 molecular weight. pure exhibits apparent weight during sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Molecular sieve chromatography under nondenaturing conditions indicates approximately 35,000, however. This result suggests that homodimer. predicted amino acid between Thr-128 Asp-160 fits consensus exhibited by many proteins bind purine nucleotides.
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