Age-dependent variations in the venom proteins of Vipera kaznakovi Nikolsky, 1909 and Vipera ammodytes (Linnaeus, 1758) (Ophidia: Viperidae)

摘要: by i and Atatur (1979) as previously described in detail (Arikan et al., 2006). Briefly, venom samples were loaded onto the polyacrylamide gels separated using a Canalco model 1200 electrophoresis apparatus. Gels stained with 0.5% Amido black to visualize protein bands densitometric curves obtained Gelman ACD-15 39430 densitometer at 500 nm.The authors received ethical permission for sampling (Ege University Animal Experiments Ethics Committee, 2010-43) special field studies (from Republic of Turkey Ministry Forestry Water Affairs).3. ResultsThe extracts Vipera kaznakovi ammodytes specimens had viscosity higher than that water. Although secretions 2 V. (16.5 30 cm total lengths) colorless, they light yellow 55-cm-long specimen. In contrast, 3 yellow.The gel photographs are presented Figure 1. According our results, proteins into 12 fractions or fraction groups shorter individuals cm), while there 13 individual (the longest one). Our comparative results electrophoretic density (protein fractions) revealed significant quantitative differences especially between individuals, similar mobility patterns numbers observed (Figures 1–4). Additionally, densities shortest differed strongly from others (Figure 1).A B C Polyacrylamide venoms different lengths. A. 16.5 cm, B. C. 55 (S: Start, junction stacking separation gels).0.225 0.200 0.175 0.150 0.125 0.100 0.075 0.050 0.025 O.D. + s 2. Gel photograph showing sample 16.5-cm-long specimen, together its tracing curve (O.D.: Optical density, S: gels).1.8 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 3. 30-cm-long curve. For further explanation, see caption 2.The 5. Similar also found ammodytes. analyses, 28.5-, 36.7-, 51.5-cm-long 13, 14, groups, respectively. (28.5-cm-long) (51.5-cm-long) 5–8).4. DiscussionMany reports literature have documented variation same population one species, during ages animal (Chippaux, 2006; Mackessy, 2010). Various on agedependent variations snake showed younger more potent terms toxicity, procoagulant defibrinating activity (Bonilla Horner, 1969; Fiero 1972; Minton, 1975; Theakston Reid, 1978; Meier Freyvogel, 1980; Glenn Straight, 1985; Furtado 1991; Tun-Pe 1995; Guercio Mackessy 2006).Several parameters affected ontogenetic development. As result increased head size so gland size, yields dry weight adult snakes generally greater those neonate juvenile this can be regarded general trend all venomous reptiles (Klauber, 1956; 1985, 1988; 2003, 2006, The S 4. 2.Figure 28.5 36.7 51.5 6. 28.5-cm-long 1.8 7. 36.7-cm-long