Detergent Lysis of Animal Tissues for Immunoprecipitation.

摘要: This protocol details protein extraction from mouse tissues for immunoprecipitation purposes and has been applied the performance of large-scale immunoprecipitations target proteins various identification associated by mass spectroscopy. The key factors in performing a successful directly relate to abundance particular tissue type whether or not embryonic, newborn, adult mouse-derived contain fibrous other insoluble material. Several types, including lung liver as well carcinomas, significant amounts that can interfere with an immunoprecipitation.