Spectrophotometric assay of clavulanic acid by reaction with imidazole

摘要: An assay method for salts of clavulanic acid is based on the absorbance at 312 nm product reaction with imidazole. The has been isolated and identified as 1-(4-aza-8-hydroxy-6-oxo)oct-2-en-1-oylimidazole. applicable to clavulanate concentrations down about 2 µg ml–1 specific intact in presence degradation products. Derivatives other compounds containing clavam nucleus also give strongly absorbing products under conditions, but penicillins little or no absorbance.