A conserved upstream element is essential for transcription of the Leishmania tarentolae mini-exon gene.

摘要: We demonstrate that the mini-exon genes of Leishmania tarentolae are individually transcribed by an enzyme pharmacologically identified as RNA polymerase II. To study transcription in these ancient eukaryotes, a stable transformation assay using episomal gene was developed. The introduced gene, which had been marked with 40 bp tag, yielded predicted tagged transcript. An upstream cis-acting element essential for site-directed mutagenesis. Block substitution mutagenesis -1 to +9 and +10 +19 regions exon results 20- 100-fold decreased levels transcript steady-state RNA. However, since two mutations resulted only 2- 3-fold decrease nascent levels, appear be affected greatest stability resulting In contrast, mutation -67/-58 region undetectable both from gene. conclude, therefore, this element, is highly conserved all species, component promoter.