Effects of colonization, luminescence, and autoinducer on host transcription during development of the squid-vibrio association

摘要: The light-organ symbiosis between the squid Euprymna scolopes and luminous bacterium Vibrio fischeri offers opportunity to decipher hour-by-hour events that occur during natural colonization of an animal's epithelial surface by its microbial partners. To determine genetic basis these events, a glass-slide microarray was used characterize transcriptome juvenile in response initiation symbiosis. Patterns gene expression were compared animals not exposed symbiont, wild-type or mutant symbiont defective either two key characters this association: bacterial luminescence autoinducer (AI) production. Hundreds genes differentially regulated as result initiation, hierarchy existed magnitude host's three features: presence > AI Putative host receptors for molecules known induce development are up-regulated light production, suggesting bioluminescence plays role preparing bacteria-induced development. Further, because transcriptional tissues context (i.e., with symbionts) differed from alone, bacteria potentiates quorum signals Comparison data those other symbioses, such germ-free/conventionalized mice zebrafish, revealed set shared may represent core ancient responses conserved throughout animal evolution.