Urine sample preparation for proteomic analysis.

摘要: Sample preparation for both environmental and more importantly biological matrices is a bottleneck of all kinds analytical processes. In the case proteomic analysis this element even important due to amount cross-reactions that should be taken into consideration. The incorporation new post-translational modifications, protein hydrolysis, or its degradation possible as side effects proteins sample processing. If protocols are evaluated appropriately, then identification such does not bring difficulties. However, if structural changes provided without sufficient attention sequence coverage will reduced could impossible. This review summarizes obstacles achievements in urine proteome using different tools mass spectrometry analysis. main aim present comprehensively idea application valuable matrix. article dedicated mainly novel cancer biomarkers discovery.