Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells
作者： S.-Y. Jeong , S.-Y. Park , Y.-H. Kim , M. Kim , S.-J. Lee
DOI: 10.1111/J.1365-2672.2007.03615.X
关键词: Metabolite 、 Annexin 、 Cytotoxicity 、 Molecular biology 、 Agarose gel electrophoresis 、 Cytotoxic T cell 、 Bacillus vallismortis 、 Biology 、 DNA fragmentation 、 Apoptosis
摘要: Aims: The objective of this research was to isolate and identify a cytotoxic marine bacterium, BIT-33, investigate the apoptosis effects its metabolite on colon cancer cells. Method Results: We isolated 93 bacteria from seawater samples. Of these, strain BIT-33 exhibited strongest activity three cells (HT-29, SW480 HCT116). Biochemical tests 16S rDNA sequencing allowed us as Bacillus vallismortis. compound B. vallismortis purified by reverse-phase high-performance liquid chromatography. Direct effect measured 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide assay. induced cells, indicated DNA fragmentation agarose gel electrophoresis, flow cytometric analysis (sub-G1 method) annexin V staining. Conclusion: purified, showed direct apoptotic in dose- time-dependent manner. Significance Impact Study: Taken together, our results suggest that could be candidate for development apoptosis-specific anti-tumour agents. This study an important source metabolites.
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