Nuclear import of the human androgen receptor

摘要: Nuclear import of the human androgen receptor was investigated by immunocytochemical analysis deletion and substitution mutants, which were transiently expressed in COS-1 cells. The signal responsible for nuclear is encoded amino-acid residues 608-625 functionally similar to bipartite nucleoplasmin nuclear-localization signal. Although subcellular distribution receptors mutated DNA-binding domain unchanged compared with wild-type receptor, presence ligand these mutations resulted part population forming clusters. Depending on or absence localization signal, clusters formed nucleus cytoplasm, respectively. Expression different cell lines revealed a cell-line-specific unliganded receptor. predominantly when HeLa cells, whereas mainly cytoplasmic staining observed it In hormone, located nucleus, independent line that expressing Anti-androgens various steroid hormones induced dose-dependent way, without activating transcription an androgen-regulated reporter gene. This indicates inability tested compounds activate not due inhibited import.