Activation of signal transduction in platelets by the tyrosine phosphatase inhibitor pervanadate (vanadyl hydroperoxide).
作者： K M Pumiglia , L F Lau , C K Huang , S Burroughs , M B Feinstein
DOI: 10.1042/BJ2860441
关键词: Tyrosine 、 Biochemistry 、 Tyrosine-kinase inhibitor 、 Protein kinase C 、 Tyrosine phosphorylation 、 Protein kinase A 、 Biology 、 Protein phosphorylation 、 Protein tyrosine phosphatase 、 Phosphorylation
摘要: The protein tyrosine phosphatase (PTPase) inhibitor pervanadate (vanadyl hydroperoxide) stimulated phosphorylation 29-fold more than did thrombin in intact and saponin-permeabilized platelets. Increased preceded, or was coincident with, a fall PtdIns(4,5)P2 levels, production of PtdIns(3,4)P2 phosphatidic acid, mobilization intracellular Ca2+, stimulation kinase C-dependent phosphorylation, secretion dense alpha-granules, increased actin polymerization, shape change aggregation which required fibrinogen mediated by surface expression GPIIb-IIIa. RG 50864 totally prevented induction pervanadate, as well all other responses measured; contrast, the inactive structural analogue, tyrphostin #1, had no effect. Dense-granule induced C activity; however, alpha-granule were independent C. In platelets phospholipase activity GTP-independent GTP-dependent mechanisms respectively. We conclude that PTPases are important regulators signal transduction
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sci-hub.se 参考文章(55)
SJ Shattil, M Cunningham, JA Hoxie, Detection of activated platelets in whole blood using activation- dependent monoclonal antibodies and flow cytometry Blood. ,vol. 70, pp. 307- 315 ,(1987) , 10.1182/BLOOD.V70.1.307.307
S W Jones, R L Erikson, V M Ingebritsen, T S Ingebritsen, Phosphotyrosyl-protein phosphatases Journal of Biological Chemistry. ,vol. 264, pp. 7747- 7753 ,(1989) , 10.1016/S0021-9258(18)83298-7
R M Lyall, A Zilberstein, A Gazit, C Gilon, A Levitzki, J Schlessinger, Tyrphostins inhibit epidermal growth factor (EGF)-receptor tyrosine kinase activity in living cells and EGF-stimulated cell proliferation. Journal of Biological Chemistry. ,vol. 264, pp. 14503- 14509 ,(1989) , 10.1016/S0021-9258(18)71707-9
S P Halenda, G B Zavoico, M B Feinstein, Phorbol esters and oleoyl acetoyl glycerol enhance release of arachidonic acid in platelets stimulated by Ca2+ ionophore A23187. Journal of Biological Chemistry. ,vol. 260, pp. 12484- 12491 ,(1985) , 10.1016/S0021-9258(17)38897-X
S Kadota, I G Fantus, G Deragon, H J Guyda, B I Posner, Stimulation of insulin-like growth factor II receptor binding and insulin receptor kinase activity in rat adipocytes. Effects of vanadate and H2O2. Journal of Biological Chemistry. ,vol. 262, pp. 8252- 8256 ,(1987) , 10.1016/S0021-9258(18)47556-4
Flavia O'Rourke, George B. Zavoico, Laurens H. Smith, Maurice B. Feinstein, Stimulus-response coupling in a cell-free platelet membrane system FEBS Letters. ,vol. 214, pp. 176- 180 ,(1987) , 10.1016/0014-5793(87)80037-6
M O Walderhaug, R L Post, G Saccomani, R T Leonard, D P Briskin, Structural relatedness of three ion-transport adenosine triphosphatases around their active sites of phosphorylation. Journal of Biological Chemistry. ,vol. 260, pp. 3852- 3859 ,(1985) , 10.1016/S0021-9258(19)83702-X
C.A. Hillery, S.S. Smyth, L.V. Parise, Phosphorylation of human platelet glycoprotein IIIa (GPIIIa). Dissociation from fibrinogen receptor activation and phosphorylation of GPIIIa in vitro. Journal of Biological Chemistry. ,vol. 266, pp. 14663- 14669 ,(1991) , 10.1016/S0021-9258(18)98736-3
L F Brass, M Laposata, H S Banga, S E Rittenhouse, Regulation of the phosphoinositide hydrolysis pathway in thrombin-stimulated platelets by a pertussis toxin-sensitive guanine nucleotide-binding protein. Evaluation of its contribution to platelet activation and comparisons with the adenylate cyclase inhibitory protein, Gi. Journal of Biological Chemistry. ,vol. 261, pp. 16838- 16847 ,(1986) , 10.1016/S0021-9258(19)75964-X
Ronald Kriz, Lih-Ling Lin, Lisa Sultzman, Christine Ellis, Carl-Henrik Heldin, Tony Pawson, John Knopf, Phospholipase C isozymes: structural and functional similarities. Ciba Foundation symposium. ,vol. 150, pp. 112- 127 ,(1990) , 10.1002/9780470513927.CH8