Modulation of the transient receptor potential channel TRPA1 by phosphatidylinositol 4,5-biphosphate manipulators.

摘要: The transient receptor potential channel of the ankyrin-binding repeat subfamily, TRPA1, is a Ca2+-permeable non-selective cation that depolarizes plasma membrane and causes Ca2+ influx. A typical feature TRPA1 its rapid desensitization following activation by agonists such as mustard oil (MO), cinnamaldehyde, high intracellular concentration. In whole-cell recordings on Chinese hamster ovary (CHO) cells expressing was delayed when phosphatidylinositol 4,5-biphosphate (PIP2) supplemented via patch pipette, whereas PIP2 scavenger neomycin accelerated desensitization. Preincubation with PI-4 kinase inhibitor wortmannin reduced both constitutive channels activity response to MO. Run down also Mg2+ concentrations, chelating 10 mM ethylenedinitrilotetraacetic acid (EDTA) increased basal activity. inside-out patches, we observed run activity, which could be prevented application diC8-PIP2 or 2 mM Mg-ATP but not Na2-ATP cytosolic side excised patches. isolated trigeminal ganglion neurons, preincubation resulted in inhibition endogenous Taken together, our data indicate modulates albeit lesser extent than other known PIP2-dependent TRP channels, tools modifying content often have direct effects this channel.