Optogenetic control of receptors reveals distinct roles for actin- and Cdc42-dependent negative signals in chemotactic signal processing

摘要: Abstract During chemotaxis, neutrophils use cell surface G-Protein Coupled Receptors (GPCRs) to detect chemoattractant gradients1–4. The downstream signaling system is wired with multiple feedback loops that amplify weak inputs and promote spatial separation of front rear activities1, 5–8. Positive could rapid signal spreading9, yet information from the receptors transmitted high fidelity, enabling detection small differences in concentration across cell1. How transduction network achieves amplification while preserving remains unclear. GTPase Cdc42 a cell-front polarity coordinator predictive turning, suggesting an important role processing10. To directly measure flow Cdc42, we paired zebrafish parapinopsina, optogenetic GPCR allows reversible ON/OFF receptor control spectrally compatible red/far red FRET biosensor. Using this new toolkit, show positive negative signals G-proteins shape rapid, dose-dependent response. Furthermore, F-actin itself provide two distinct limit duration spread activation, maintaining output local originating receptors.