Proposed Arrangement of Proteins Forming a Bacterial Type II Polyketide Synthase
作者： Gaetano Castaldo , Jurica Zucko , Sibylle Heidelberger , Dušica Vujaklija , Daslav Hranueli
DOI: 10.1016/J.CHEMBIOL.2008.09.010
关键词: Cyclase 、 Anthraquinones 、 Stereochemistry 、 Biology 、 Protein–protein interaction 、 Polyketide synthase 、 Protein subunit 、 ATP synthase 、 Protein structure 、 Biochemistry 、 Acyltransferase 、 Clinical biochemistry 、 Molecular medicine 、 Molecular biology 、 Pharmacology 、 Drug discovery 、 General Medicine
摘要: Aklanonic acid is synthesized by a type II polyketide synthase (PKS) composed of eight protein subunits. The network interactions within this complex was investigated using yeast two-hybrid system, coaffinity chromatography and two different computer-aided docking simulations. Results suggest that the ketosynthase (KS) alpha beta subunits interact with each other, KSalpha subunit also probably interacts malonyl-CoA:ACP acyltransferase (DpsD), forming putative minimal synthase. We speculate DpsD may physically inhibit priming reaction, allowing choice propionate rather than acetate as starter unit. structural role for cyclase (DpsY) in maintaining overall integrity complex.
core.ac.uk
bristol.ac.uk
elsevier.com
irb.hr 
sci-hub.se 参考文章(72)
Peter J. Kramer, Robert J. X. Zawada, Robert McDaniel, C. Richard Hutchinson, David A. Hopwood, Chaitan Khosla, RATIONAL DESIGN AND ENGINEERED BIOSYNTHESIS OF A NOVEL 18-CARBON AROMATIC POLYKETIDE Journal of the American Chemical Society. ,vol. 119, pp. 635- 639 ,(1997) , 10.1021/JA962888Q
Sven-Eric Wohlert, Evelyn Wendt-Pienkowski, Wuli Bao, C. Richard Hutchinson, Production of aromatic minimal polyketides by the daunorubicin polyketide synthase genes reveals the incompatibility of the heterologous DpsY and JadI cyclases. Journal of Natural Products. ,vol. 64, pp. 1077- 1080 ,(2001) , 10.1021/NP010067F
Galina Florova, Galina Kazanina, Kevin A. Reynolds, Enzymes Involved in Fatty Acid and Polyketide Biosynthesis in Streptomyces glaucescens: Role of FabH and FabD and Their Acyl Carrier Protein Specificity† Biochemistry. ,vol. 41, pp. 10462- 10471 ,(2002) , 10.1021/BI0258804
Thomas B. Thompson, Kinya Katayama, Kenji Watanabe, C. Richard Hutchinson, Ivan Rayment, Structural and Functional Analysis of Tetracenomycin F2 Cyclase from Streptomyces glaucescens A TYPE II POLYKETIDE CYCLASE Journal of Biological Chemistry. ,vol. 279, pp. 37956- 37963 ,(2004) , 10.1074/JBC.M406144200
Yi Tang, Taek Soon Lee, Chaitan Khosla, Engineered Biosynthesis of Regioselectively Modified Aromatic Polyketides Using Bimodular Polyketide Synthases PLOS Biology. ,vol. 2, ,(2004) , 10.1371/JOURNAL.PBIO.0020031
Christian Hertweck, Andriy Luzhetskyy, Yuri Rebets, Andreas Bechthold, Type II polyketide synthases: gaining a deeper insight into enzymatic teamwork Natural Product Reports. ,vol. 24, pp. 162- 190 ,(2007) , 10.1039/B507395M
C R Hutchinson, A L Colombo, Genetic engineering of doxorubicin production in Streptomyces peucetius : a review Journal of Industrial Microbiology & Biotechnology. ,vol. 23, pp. 647- 652 ,(1999) , 10.1038/SJ.JIM.2900673
Jodi R Parrish, Keith D Gulyas, Russell L Finley, Yeast two-hybrid contributions to interactome mapping Current Opinion in Biotechnology. ,vol. 17, pp. 387- 393 ,(2006) , 10.1016/J.COPBIO.2006.06.006
John Moult, A decade of CASP: progress, bottlenecks and prognosis in protein structure prediction. Current Opinion in Structural Biology. ,vol. 15, pp. 285- 289 ,(2005) , 10.1016/J.SBI.2005.05.011
Timothy S. Hitchman, John Crosby, Kate J. Byrom, Russell J. Cox, Thomas J. Simpson, Catalytic self-acylation of type II polyketide synthase acyl carrier proteins Chemistry & Biology. ,vol. 5, pp. 35- 47 ,(1998) , 10.1016/S1074-5521(98)90085-0