Application of capacitively coupled contactless conductivity as an external detector for zone electrophoresis in poly(dimethylsiloxane) chips
作者： Péter I. Koczka , Ede Bodoki , Attila Gáspár
关键词: Thermal conductivity detector 、 Theoretical plate 、 Electrophoresis 、 Conductivity 、 Detection limit 、 Analytical chemistry 、 Microfluidics 、 Detector 、 Materials science 、 Electrode
摘要: In this work, lab-made PDMS microfluidic chips were matched to a capacitively coupled contactless conductivity detector (C(4) D) having external in-plane electrodes (eDAQ, Australia). The advantages of type C(4) D are the choice reversibly place or remove microchip onto/from and freely variate position detection (separation length) on microchip. thickness bottom layer chip was optimized achieve sensitive during electrophoretic separation. with 100 μm used platform tested by CZE mixture seven anions different types real samples. Using split-flow pressure sample injection effective length 6.5 cm, numbers theoretical plates in range 4000-6000 (63,000-93,000/m) LODs amounted 3.66-14.7 μmol/L (0.13-2.26 μg/mL) for studied anions.
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