Polymer-based microfluidic chip for rapid and efficient immunomagnetic capture and release of Listeria monocytogenes

摘要: Infections caused by foodborne pathogens such as Listeria monocytogenes pose a threat to public health while timely detection is challenging due pathogen low numbers. The development of robust and efficient sample preparation techniques crucial improve sensitivity workflow. Immunomagnetic separation using magnetic nanoparticles (MNPs) attractive, it can efficiently capture target cells. For food safety applications, platform needed rapidly process large volumes, allowing release bacteria conjugated immunomagnetic (IMNPs). Herein, we demonstrate method for bacteria-IMNPs complex based on 3D trap integrated polymeric microfluidic device. region consist dense array high-aspect ratio (3 : 1) cylindrical pillars embossed in thermoplastic polymer coated with soft ferromagnetic nickel an electroless deposition technique. This allows the generation strong switchable regions very remanence shell. We propose validate optimized configuration localized rapid MNPs IMNPs L. monocytogenes. A maximum recovery rate corresponded 91% efficiency 30% was obtained live bacteria, minimum detectable concentration ~10 cfu ml−1 1 ml volume plate-culture method. believe that flexible design low-cost fabrication proposed system will allow applications beyond water safety, including point-of-care diagnosis.