Native Chemical Ligation: SemiSynthesis of Post-translationally Modified Proteins and Biological Probes
作者： E. Vogel Taylor , B. Imperiali
DOI: 10.1007/978-3-540-70941-1_3
关键词: Native chemical ligation 、 Biochemistry 、 Peptide bond 、 Homogeneous 、 Peptide 、 Chemistry 、 Protein domain 、 Semisynthesis 、 Amino acid 、 Recombinant peptide
摘要: Native chemical ligation (NCL) is a chemoselective reaction that joins synthetic or recombinant peptide and protein fragments through native bond. Advances in generating the required components for NCL have enabled homogeneous incorporation of unnatural amino acids, fluorescent probes, other modifications into domains full-length proteins. The resulting semisynthetic proteins, which can be made multi-milligram quantities, may used biophysical biochemical studies to address challenging biological problems not accessible with traditional methods. This chapter provides an overview NCL, B. Imperiali Department Chemistry Biology, Massachusetts Institute Technology, 77 Avenue, Cambridge, MA 02139, USA, e-mail: imper@mit.edu
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