Downregulation of miR-329 promotes cell invasion by regulating BRD4 and predicts poor prognosis in hepatocellular carcinoma
作者： Jianping Zhou , Weiling Li , Jianfeng Guo , Gang Li , Fang Chen
DOI: 10.1007/S13277-015-4109-4
关键词: BRD4 、 Apoptosis 、 microRNA 、 Pathology 、 Cell 、 Downregulation and upregulation 、 Medicine 、 Cell growth 、 Cancer research 、 Hepatocellular carcinoma 、 Western blot
摘要: Increasing evidence indicates that abnormal microRNA (miRNA) expression is related to hepatocellular carcinoma (HCC) development. Our study aimed elucidate the essential role of miR-329 in HCC progression. Real-time PCR was used analyze and bromodomain containing 4 (BRD4) samples (n = 135). Cell Counting Kit-8 (CCK-8) flow cytometric analysis were investigate cell proliferation apoptosis. The transwell assay examine invasive ability. regulation mechanism confirmed by luciferase reporter western blot assays. Kaplan-Meier detect function on prognosis patients. decreased tumor Furthermore, significantly regulated invasion targeting BRD4 but had no effect Moreover, downregulation predicted poor could control via regulating may be a prognostic marker HCC.
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