Alpha-factor synchronization of budding yeast.

摘要: Publisher Summary This chapter discusses the α-factor synchronization of budding yeast. The ease with which one can synchronize populations Saccharomyces cerevisiae cells has been invaluable for studies cell cycle. Synchrony be obtained either by inducing specific arrests, or collecting small G1 on basis size, then releasing these into growth medium to undergo synchronous cycles. Every protocol its limitations, so a prudent strategy determining if process is cycle regulated employ at least two different synchrony methods. If oscillation observed through more mitotic cycles in types experiments, it unlikely that induced arrest. focuses particular method exploits natural features mating pathway S. generate population highly synchronized cells. First, rationale choosing this provided, and differences between pheromone-induced other methods are available highlighted.