Characterization of the endophytic antagonist pY11T-3-1 against bacterial soft rot of Pinellia ternata.

摘要: Aims:  Biocontrol is an emerging trend aimed at reducing chemical input while increasing plant fitness, productivity and resistance to diseases in sustainable agriculture. An antagonist, pY11T-3-1, was herein characterized for potential applications against soil-borne diseases. Methods Results: In vitro antagonistic assays, the antagonist pY11T-3-1 demonstrated able obviously reduce occurrence of soft rot disease on Pinellia ternata, potato, pepper, tomato, cucumber eggplant tubers or fruits, with higher prevention (90%) P. ternata. It showed a broad spectrum 23 tested bacterial fungal phytopathogens, which were distributed 14 genus 17 species. However, it inhibited only two seven nonpathogens. Phenotypic characterizations that similar Pseudomonas aeruginosa. Its major fatty acids 18:1 w7c (22·17%), 16:0 (20·21%), 12:0 2OH (12·45%), 16:1w7c/15 iso2OH (10·95%) 10:0 3OH (10·79%), different profile from Ps. aeruginosa. The 16S rRNA gyr B gene sequences shared 100 99% similarity Ps. aeruginosa, respectively. phylogenetic trees clustered Ps. aeruginosa. Conclusions:  as Ps. aeruginosa unique acid profile. Significance Impact Study:  With host selectivity, may provide more environmental economical alternative control P. ternata, needs further investigation.