Method of isolating nucleic acid targets

摘要: The invention provides efficient methods of isolating specific nucleic acid targets to obtain information from target sequences in a relatively short time period. DNA or cDNA is enzymatically digested into smaller fragments, double-stranded linkers are added onto the ends fragments flank each fragment with known sequence. mixed an oligonucleotide probe that bound marker and contains conserved sequence interest. hybridize through nucleotide base pair complementation become indirectly connected marker. These captured using capture agent specifically recognizes treated prevent non-specific binding. Captured typically cloned prior sequencing. may also be amplified PCR increase efficiency cloning.