Targeting vector construction method for site-directed integration of exogenous gene into GAPDH gene and its application
作者： Li Changchun , Fu Liangliang , Han Xiaosong , Xie Shengsong , Xiong Youcai
DOI:
关键词: Cas9 、 Subgenomic mRNA 、 Genome 、 Multiple cloning site 、 Stop codon 、 Biology 、 Gene 、 GAPDH Gene 、 CRISPR 、 Computational biology
摘要: The invention discloses a targeting vector construction method for site-directed integration of an exogenous gene into GAPDH and its application. takes the termination codon upstream downstream sequences as left right homologous arms, arm, 2A sequence, sequence arm are sequentially connected, subjected to reverse insertion multiple cloning site eukaryotic expression vector. is co-transfected porcine cell with CRISPR/Cas9 cleavage containing sgRNA specifically gene, can be site-integrated gene. target provided by broaden range sites in pig genome, lay foundation preparing efficient stable genome.
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