Oral administration of human insulin to NOD mice generates CD4+ T cells that suppress adoptive transfer of diabetes.

摘要: Oral administration of porcine insulin has been shown to be effective in preventing the spontaneous occurrence diabetes Non-Obese Diabetic (NOD) mouse model. In present study, we demonstrate that feeding 6-week-old female mice with 20 units human every 2-3 days for 30 induces an active mechanism suppression through generation regulatory T cells. Adult irradiated NOD males i.v. injected 5 x 10(6) cells from spleens diabetic donors and same number insulin-fed animals had less successful transfer than controls (4/15 vs. 8/16, P < 0.001). Protection clinical was associated a reduction severe insulitis (16.4 +/- 3.6% 52.3 12.8%, = 0.023). However, more 85% islets were inflamed. Feeding 15 reduced magnitude this protection since transfers after 1 month comparable (12/17 14/17) despite significant delay onset (P No difference contribution cell subsets noted by cytofluorometry treated animals. When co-injected diabetogenic cells, only purified CD4+ able 3/12 became 36 comparison 3/6 group PBS-fed animals, or 5/6 CD8+ cells.(ABSTRACT TRUNCATED AT 250 WORDS)