Methods for in vitro expansion and transdifferentiation of human pancreatic acinar cells into insulin-producing cells
作者： David W. Scharp , Paul P. Latta , Catherine Mcintyre , Margaret Coutts , Neil Robbins
DOI:
关键词: In vitro 、 Chemically defined medium 、 Insulin 、 Phenotype 、 Cell culture 、 Cell 、 Transdifferentiation 、 Pancreatic acinar cells 、 Biology 、 Cell biology
摘要: This invention relates, e.g., to a method for expanding mammalian acinar cells, comprising culturing the cells in cell culture system medium and attachment surface, under conditions wherein undergo 3-4 fold expansion together with transdifferentiation into modified phenotype (IP cells) showing characteristics of liver cells. The also relates transforming these IP insulin-producing vitro, novel, defined medium. Also disclosed are suitable media performing methods, isolated having and/or produced by kits methods.
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