Development and validation of a new high-throughput method to investigate the clonality of HTLV-1-infected cells based on provirus integration sites

摘要: Transformation and clonal proliferation of T-cells infected with human T-cell leukemia virus type-I (HTLV-1) cause adult leukemia. We took advantage next-generation sequencing technology to develop internally validate a new methodology for isolating integration sites estimating the number cells in each HTLV-1-infected clone (clone size). Initial analysis was performed DNA samples from individuals. then used appropriate controls known clonality status confirm accuracy our system, which indeed had least errors among currently available techniques. Results suggest potential clinical biological applications method.