Mendelian transmission, transgene dosage and growth phenotype in transgenic tilapia (Oreochromis hornorum) showing ectopic expression of homologous growth hormone.
作者： Rebeca Martı́nez , Amilcar Arenal , Mario Pablo Estrada , Fidel Herrera , Vivian Huerta
DOI: 10.1016/S0044-8486(98)00451-7
关键词: Genetics 、 Embryo 、 Microinjection 、 Gonad 、 Transgene 、 Molecular biology 、 Oreochromis 、 Transformation (genetics) 、 Biology 、 Ectopic expression 、 Tilapia
摘要: Abstract Gene transfer has offered a new tool for the development of improved fish strains aquaculture. However, characterization is required before these can be introduced into national aquaculture programs. Transgenic tilapia (O. hornorum urolepis) were produced by microinjection early embryos transgene containing growth hormone (tiGH) cDNA under regulatory sequences derived from human cytomegolovirus (CMV). A male 1 copy/cell was selected to establish transgenic line. The transmitted F1–F4 generations in Mendelian fashion. Previous studies showed ectopic, low level expression tiGH brain, heart, gonad, liver and muscle cells tilapia. Biochemical analyses indicated lower levels cholesterol, free alanine aspartic acid animals. Four month old homozygous (F2+/+) heterozygous (F2−/+) non-transgenic siblings (NTRANSGENICS=14; NCONTROLS=11; NF2−/+=8; NF2+/+=6) studied 3 months grown communally same pond. (F2−/++F2+/+), F2−/+, F2+/+ progeny larger than at P=0.009, P=0.005 P=0.07 (Student t-test), respectively, suggesting transgene-dosage effect. These results indicate stable germ line transformation this fast-growing
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