Extracorporeal shock wave therapy ameliorates cyclophosphamide-induced rat acute interstitial cystitis though inhibiting inflammation and oxidative stress-in vitro and in vivo experiment studies.

摘要: Background: We investigated whether extracorporeal shock wave (ECSW) therapy can attenuate cyclophosphamide (CYP)-induced acute interstitial cystitis (AIC) in rats. Methods and Results: Eighteen male-adult Sprague-Dawley rats were equally divided into group 1 (sham control), 2 (AIC induced by 150 mg/kg CYP intra-peritoneal injection) 3 + ECSW 200 impulses at 0.11 mJ/mm2 to the urinary bladder 24 h after treatment). Smooth-muscle cells co-culture with menadione (25 µM) without treatment was performed. Western-blot results demonstrated that significant attenuated oxidative stress inflammatory reactions this in-vitro studies (all p < 0.001). 24-hour urine amount microscopic findings of red-blood-cell count (i.e., hematuria) higher than groups 3, significantly The levels albumin interleukin-6 showed an identical pattern hematuria among all three cellular mRNA expressions macrophage migration inhibitory factor (MIF)+, CD74+, CD68+, substance p+, Cox-2+ tissue exhibited 0.0001). integrity epithelial layer collagen-deposition area as stained Sirius red displayed opposite (p protein expression IL-12, iNOS, TNF-α, NF-κB, MMP-9, NOX-1, NOX-2, RANTES, Oxyblot 0.01). Conclusion: markedly CYP-induced AIC through inhibitions inflammation stress.