Cellular compartmentalization of internalized paramagnetic liposomes strongly influences both T1 and T2 relaxivity.

摘要: In recent years, numerous Gd(3+)-based contrast agents have been developed to enable target-specific MR imaging of in vivo processes at the molecular level. The combination powerful and amplification strategies, aimed increasing agent dose target site, is an often-used strategy improve sensitivity biomarker detection. One such mechanism a disease-specific cell membrane receptor that can undergo multiple rounds internalization following ligand binding thus shuttle sizeable amount into cell. An example alpha(nu)beta(3) integrin. goal this study was investigate consequences approach for T(1)- T(2)-shortening efficacy paramagnetic agent. Cultured endothelial cells were incubated with liposomes conjugated cyclic RGD-peptide by means integrin receptor. Non-targeted served as control. This showed targeting dramatically increased uptake liposomes. strategy, however, strongly influenced both longitudinal transverse relaxivity internalized