Reactive astrocytosis in feline neonatal hydrocephalus: acute, chronic, and shunt-induced changes

摘要: Reactive astrocytosis has been implicated in injury and recovery patterns associated with hydrocephalus. To investigate temporal changes astrogliosis during the early progression of hydrocephalus, after shunting, long-term ventriculomegaly, glial fibrillary protein (GFAP) levels were analyzed a feline model. Obstructive hydrocephalus was induced 10-day-old kittens by intracisternal injections 25% kaolin. Acute non-shunted animals killed 15 days post-kaolin injection to represent pre-shunt condition. Shunt-treated received ventriculoperitoneal shunts post-injection 10 or 60 days later short- periods. Chronic untreated had Ommaya reservoirs implanted post-kaolin, which tapped intermittently until they later. Ventriculomegaly monitored neuroimaging before shunting at death. RNA total from primary visual cortex Northern Western blotting. GFAP for acute chronic hydrocephalic 77% 247% (p < 0.01) 659% (p < 0.05) 871% higher than controls, respectively. Shunted short-term demonstrated mismatch levels, expression decreasing 26% increasing 335% (p < 0.01). exhibited 201% 357% above normal, These results indicate that reactive astrocytic response continues rise dramatically suggesting ongoing gliosis potential damage. Shunting partially ameliorates continuation astrogliosis, but does not completely reverse this inflammatory reaction even long recovery.