Global Proteomic Assessment of the Classical Protein-Tyrosine Phosphatome and “Redoxome”

摘要: Protein-tyrosine phosphatases (PTPs), along with protein-tyrosine kinases, play key roles in cellular signaling. All Class I PTPs contain an essential active site cysteinyl residue, which executes a nucleophilic attack on substrate phosphotyrosyl residues. The high reactivity of the catalytic cysteine also predisposes to oxidation by reactive oxygen species, such as H2O2. Reversible PTP is emerging important regulatory mechanism and might contribute diseases cancer. We exploited these unique features enzymology develop proteomic methods, broadly applicable cell tissue samples, that enable comprehensive identification quantification expressed classical (PTPome) oxidized subset PTPome (oxPTPome). find mouse human cells tissues, including cancer cells, display distinctive PTPomes oxPTPomes, revealing additional levels complexity regulation phosphorylation normal malignant cells.