Linoleic acid and α‐linolenic acid inhibit conjugative transfer of an IncX4 plasmid carrying mcr‐1

作者: G. Li , L.‐J. Xia , S.‐Y. Zhou , X.‐R. Wang , C.‐Y. Cui

DOI: 10.1111/JAM.14885

关键词: Protein sequencingBiochemistryBacteriaHomology (biology)Linoleic acidGeneGene expressionMCR-1PlasmidChemistry

摘要: Aims The aim of this study was to determine the effects unsaturated fatty acids on clinical plasmids. Methods and results Two acids, linoleic acid (LA) α-linolenic (ALA) at final concentration 0, 0·03, 0·3 3 mmol l-1 , respectively, were used assess conjugative transfer a mcr-1-harbouring plasmid pCSZ4 (IncX4) in conjugation experiment. inhibitory mechanisms analysed by molecular docking gene expression virB11 quantitated qRT-PCR. Target diversity carried out TrwD/VirB11 homology protein sequence prediction analysis. Our showed that LA ALA inhibit binding amino residues (Phe124 Thr125) VirB11 with dose-dependent effects. levels also significantly inhibited treatment. Protein analysis revealed wide distribution TrwD/VirB11-like genes among over 37 classes plasmids originated from both Gram-negative Gram-positive bacteria. Conclusions This demonstrates representing may be potentially acids. Significance impact work reported here provides additional support for application curbing spread multiple

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