Genetic Manipulation of Myxobacteria

摘要: Genome sequencing of a few myxobacteria has revealed genes for the biosynthesis unidentified secondary metabolites. Considering need new drugs to treat variety diseases, further exploration is imperative. Tools genetic manipulation are important identifying and engineering strains maximize production Several developments have enabled heterologous gene expression in myxobacteria, including construction development regulated promoters identification strong constitutive promoters. While most tools were first developed model myxobacterium Myxococcus xanthus, many been applied Sorangium cellulosum, Stigmatella aurantiaca, lesser-known Chondromyces crocatus, Cystobacter fuscus, Angiococcus disciformis, Corallococcus macrosporus. This chapter describes these hopes facilitate increased use applications biotechnology drug discovery. Electroporation become common technique introduce DNA into species M. S. C. A. Mutagenesis fundamental any manipulation. Transposon mutagenesis critical myxobacterial genetics due ease mutation identification. Genetic mapping linkage analysis may be performed using generalized transduction or genomic transformation by electroporation xanthus. systems used The tetR-tetA regulatory system from Escherichia coli was recently engineer another promoter