Human Apolipoprotein B

摘要: Human liver apo B-100 has been cloned in plasmid and phage λgt-11 expression vectors cDNA clones were identified by screening with monospecific anti-apo B antibodies synthetic oligonucleotides based on peptides isolated sequenced from B-100. Overlapping containing the entire mRNA sequenced. All previously their locations defined. Northern blot analysis utilizing radiolabeled probes revealed that apoB-100 is 14.1 kb size. On contrary, two distinct molecular species of are being produced small intestine. The larger-molecular-weight migrate at same position as mRNA. smaller 7.5 size, most likely codes for B-48. Hybridization nucleic acid sequence studies carried out to define common domains mRNA-specific domains. Since a single-copy gene, we propose mechanism generation B-48 through differential splicing precursor RNA transcript. We have also localized gene p23 → pter region short arm chromosome 2 filter hybridization human-mouse hybrid cell DNAs. initiated organization patients abetalipoproteinemia. Studies unrelated kindreds no major deletions or insertions protein present cells abetalipoproteinemia patients.