Partitioning of large and minichromosomes in Trypanosoma brucei
作者： Klaus Ersfeld , Keith Gull
DOI: 10.1126/SCIENCE.276.5312.611
关键词: Kinetochore 、 Chromosome 、 Nuclear gene 、 Trypanosoma brucei 、 Genetics 、 Minichromosome 、 Spindle apparatus 、 Population 、 Biology 、 Mitosis
摘要: The Trypanosoma brucei nuclear genome contains about 100 minichromosomes of between 50 to 150 kilobases and 20 chromosomes 0.2 6 megabase pairs. Minichromosomes contain nontranscribed copies variant surface glycoprotein (VSG) genes are thought expand the VSG gene pool. Varying expression allows parasite avoid elimination by host immune system. mechanism inheritance T. was investigated in situ hybridization combination with immunofluorescence. minichromosome population segregated precision, association central intranuclear mitotic spindle. However, their positional dynamics differed from that large chromosomes, which were partitioned kinetochore microtubules.
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