31P NMR and saturation transfer studies of the effect of Pb2+ on cultured osteoblastic bone cells.

摘要: Abstract The mechanism of lead toxicity at the cellular level remains unknown, although an effect on intracellular Ca2+ has been described. Since bone is a major target for lead, we have investigated bioenergetic rates and free Mg2+ concentration in cultured osteoblastic cells. Using 31P NMR saturation transfer technique detected sizable (18%) from gamma ATP to Pi perfused osteosarcoma cell line, Ros 17/2.8, found large (greater than 82%) reduction Pi----ATP rate upon treatment with 10 microM Pb2+. NMR-measured unidirectional was much greater net synthesis through glycolysis oxidative phosphorylation. By using iodoacetate that it catalyzed by glycolytic enzyme couple glyceraldehyde-3-phosphate dehydrogenase/phosphoglycerate kinase. as measured lactate production phosphorylation O2 consumption were be significantly decreased 18 74%, respectively, treatment. In addition, chemical shifts resonances, significant 21% Pb2+ observed lead-induced synthesis/utilization decrease may contribute impairment formation during intoxication.