Research on the mechanism of endothelin inflammatory effects on human mesangial cells.
作者： Jingmei Lin , Yijuan Sun , Haiyan Wang , Yipu Chen , Anyu Zhou
DOI:
关键词: Endothelin receptor 、 Northern blot 、 Mitogen-activated protein kinase 、 Tumor necrosis factor alpha 、 Cell 、 Messenger RNA 、 Cell adhesion 、 Radioimmunoassay 、 Molecular biology 、 Chemistry
摘要: OBJECTIVE To investigate the mechanism of endothelin (ET) inflammatory effects on human mesangial cells (HMC). METHODS The following experiments were performed cultured HMC after ET-1 stimulation: (1) expression tumor necrosis factor-alpha (TNF alpha), interleukin-1 beta (IL-1 beta), intercellular adhesion molecule-1 (ICAM-1), vascular cell (VCAM-1) and endothelin-1 (ET-1) itself messenger ribonucleic acid (mRNA) was determined by Northern Blot analysis; (2) TNF alpha concentration tested with radioimmunoassay; IL-1 activity assayed enhancement thymocyte proliferation in response to mitogen; surface ICAM-1 VCAM-1 measured enzyme linked immunoadsorbent assay (ELISA) analysis. RESULTS (10(-7) mol/L) induced changes HMC: up-regulation mRNA protein; (3) mRNA. However, protein not changed. CONCLUSIONS can stimulate produce alpha, VCAM-1, thereby induce effects. also up-regulate itself, so as amplify So, is actually an mediator may play important role pathogenesis glomerulonephritis.
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