Phytohemagglutinin-induced skin swelling in birds : histological support for a classic immunoecological technique

摘要: Summary 1Measurements of phytohemagglutinin (PHA)-induced tissue swelling are arguably the most popular surrogates for immunocompetence in wild birds. It is largely unresolved, however, whether basic assumption underlying these measures valid, particularly more represents a ‘better’ or ‘stronger’ cell-mediated immune response. 2In this study we took first step towards such validation by characterizing cell infiltration over time into wing-webs (patagia) PHA-challenged House Sparrows (Passer domesticus). Relative to saline-injected wing-webs, PHA-injected displayed intensive many types, including basophils, eosinophils, heterophils, lymphocytes, macrophages and thrombocytes. The abundance types changed course response (6–48 h post-injection). Peak varied depending on type. At several points, significant correlations between numbers some (particularly heterophils) degree were detected. 3Together, data indicate that PHA-induced related heightened activity Sparrows, but also PHA species dynamic involves both innate adaptive components system. We thus caution against interpreting larger swellings as ‘greater immunocompetence’, given complex nature response.