Somatic embryogenesis in the medicinal legume Desmodium motorium (Houtt.) Merr.
作者： B. Chitra Devi , V. Narmathabai
DOI: 10.1007/S11240-011-9937-3
关键词: Botany 、 Desmodium 、 Cotyledon 、 Abscisic acid 、 Embryogenesis 、 Biology 、 Embryo 、 Murashige and Skoog medium 、 Somatic embryogenesis 、 Germination
摘要: An efficient protocol was established for regeneration of Desmodium motorium via somatic embryogenesis. Embryogenic calli were induced from cotyledon segments (6 mm, 16 days old) lacking embryo axis, excised seedlings grown in vitro on Murashige and Skoog (MS) medium supplemented with indole-3-acetic acid (IAA) (2.9 μM) in combination 6-benzyladenine (BA) (4.44 8.88 μM). Differentiation embryogenic into globular heart-shaped embryos achieved transfer to hormone-free MS medium. When incubated 4 days BA (8.88 μM), 95% the matured torpedo cotyledonary stages minimum (10%) abnormalities. Modified basal without hormones containing half-strength macronutrients 0.88 M sucrose suitable germination mature embryos. Regenerated plantlets successfully transferred earthen pots survival rate 50%. Secondary embryogenesis observed when pre-existing at cultured various concentrations BA, adenine sulphate (AdS) abscisic (ABA) individually.
springer.com
sci-hub.se 参考文章(82)
W. A. Parrott, R. E. Durham, M. A. Bailey, Somatic Embryogenesis in Legumes Somatic Embryogenesis and Synthetic Seed II. pp. 199- 227 ,(1995) , 10.1007/978-3-642-78643-3_17
G. Lakshmi Sita, Abha Khandelwal, M. S. Shaila, P. Venkatachalam, N. Geetha, Induction of direct somatic embryogenesis and plant regeneration from mature cotyledon explants of Arachis hypogaea L Current Science. ,vol. 77, pp. 269- 273 ,(1999)
David A. Stuart, Janet Nelsen, Carol M. McCall, Steven G. Strickland, Keith A. Walker, PHYSIOLOGY OF THE DEVELOPMENT OF SOMATIC EMBRYOS IN CELL CULTURES OF ALFALFA AND CELERY Biotechnology in Plant Science#R##N#Relevance to Agriculture in the Eighties. pp. 35- 47 ,(1985) , 10.1016/B978-0-12-775310-2.50009-0
S. A. Merkle, W. A. Parrott, B. S. Flinn, Morphogenic Aspects of Somatic Embryogenesis Springer Netherlands. pp. 155- 203 ,(1995) , 10.1007/978-94-011-0485-2_5
J. Austin Burns, Hazel Y. Wetzstein, Embryogenic cultures of the leguminous tree Albizia julibrissin and recovery of plants Plant Cell Tissue and Organ Culture. ,vol. 54, pp. 55- 59 ,(1998) , 10.1023/A:1006024322446
K. Ahmad, V. K. Singh, M. K. Siddiqui, R. K. Sharma, J. N. Govil, Aminuddin, R. D. Girach, Ethnomedicinal plants in skin care from Orissa, India. Studium Press LLC. pp. 11- 78 ,(2007)
S Girija, A Ganapathi, G Ananthakrishnan, Somatic embryogenesis in Vigna radiata (L.) Wilczek. Indian Journal of Experimental Biology. ,vol. 38, pp. 1241- 1244 ,(2000)
J. N. Govil, S. Singh, V. K. Singh, Phytochemistry and pharmacology. Studium Press, LLC. ,(2003)
In vitro embryogenesis in plants Kluwer Academic Publishers. ,(1995) , 10.1007/978-94-011-0485-2
Y. P. S. Bajaj, Somatic Embryogenesis and Synthetic Seed I ,(1995)