Promoting activities of butylated hydroxyanisole and butylated hydroxytoluene on 2-stage urinary bladder carcinogenesis and inhibition of γ-glutamyl transpeptidase-positive foci development in the liver of rats

摘要: Butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) were evaluated for possible promoting activity urinary bladder carcinogenesis in male F344 rats initiated by N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN). The treated with 0.01 or 0.05% BBN the drinking water 4 weeks then administered 2% BHA 1% BHT diet 32 weeks. Surviving killed at end of week 36 experiment. incidences cancer papilloma average number cancers, papillomas papillary nodular hyperplasias (PN hyperplasias) per 10 cm basement membrane significantly increased group receiving following initiation compared given only. also these lesions bladder, but not BBN. ability four antioxidants, BHA, BHT, sodium L-ascorbate (ascorbate) ethoxyquin, to promote induction gamma-glutamyltranspeptidase (gamma-GT)-positive foci diethylnitrosamine (DENA) liver was tested. Rats a single i.p. injection 200 mg/kg body weight DENA, 2 later animals exposed 5% ascorbate respectively, 6 All subjected partial hepatectomy 3. gamma-GT-positive groups fed either ethoxyquin after DENA decreased control group. These findings show that are promoters BBN, other antioxidants inhibit liver.