Observation of ice-like water layers at an aqueous protein surface
作者： Konrad Meister , Simona Strazdaite , Arthur L. DeVries , Stephan Lotze , Luuk L. C. Olijve
关键词: Sum frequency generation spectroscopy 、 Aqueous solution 、 Freezing point 、 Antifreeze protein 、 Properties of water 、 Chemical engineering 、 Crystallography 、 Crystallization 、 Surface protein 、 Femtosecond 、 Chemistry
摘要: We study the properties of water at surface an antifreeze protein with femtosecond sum frequency generation spectroscopy. find clear evidence for presence ice-like layers ice-binding site in aqueous solution temperatures above freezing point. Decreasing temperature to biological working (0 °C −2 °C) increases amount water, while a single point mutation is observed completely disrupt character and eliminate activity. Our observations indicate that not itself but ordered are responsible recognition binding ice.
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