Molecular cell biology: Epigenetic gene silencing in plants

摘要: The highly organized arrangement of the DNA in cell nucleus and structurally defined compartments within are important prerequisites for a timely spatially correct gene expression functioning eukaryotic cell. Since it is known that nuclear wrapped up into nucleoprotein chromatin complex forming periodically arranged nucleosomes, factors involved stabilization remodeling during different stages activity have attracted lot interest (Reyes et al. 2002). During 1980s, much emphasis was laid on unraveling structure regulation transcription catalyzed by RNA polymerases mediated stimulated factors. In last decade, structural organization cycle underwent revival research activity, silencing investigated more detail. central component chromatin, nucleosome, contains conserved histone proteins, which now to be subject wide range post-translational modifications act as recognition sites binding chromatin-associated Earlier already that, addition these (Loidl 2004), methylation could also dramatic influence (Bowler 2004). Detailed cytological investigations with improved multicolor fluorescent in-situ hybridization (FISH) techniques (Lichter 1997) elucidated higher order chromosomes; recently information has been gained regarding this organization, not only at chromosome level mitotic or meiotic cell, but mainly euand heterochromatic interphase stage (Tariq Paszkowski Recent studies demonstrated many aspects plant development accompanied heritable changes