In vitro 1H NMR spectroscopy shows an increase in N-acetylaspartylglutamate and glutamine content in the hippocampus of amygdaloid-kindled rats.

摘要: We examined energy metabolism and amino acid content in the hippocampus of amygdaloid-kindled rats using 1H NMR spectroscopy. Three weeks after last stage 5 seizure, kindled were killed by microwave irradiation. The was dissected out subjected to MeOH/CHCl3 extraction. All 1H spectra analyzed quantify absolute concentrations a non-linear least squares method, combined with prior knowledge chemical shifts. Saturation effects compensated for T1 measurement each component. Levels metabolism-related compounds, phosphocreatine, creatine, glucose succinate same both sham controls. Lactate concentration had tendency increase, although this not statistically significant. When compared controls, levels aspartate, glutamate, glycine glutamine, as well GABA inositol, increased ipsilateral but contralateral hippocampus. In contrast, taurine, alanine threonine unchanged. Finally, N-acetylaspartylglutamate elevated, whereas N-acetyl-l-aspartate unaltered animals. Our results suggest that amygdala kindling may affects metabolism, metabolism.