Effects of dietary fish oil on plasma high density lipoprotein. Electron spin resonance and fluorescence polarization studies of lipid ordering and dynamics.

摘要: Dietary fish oils are implicated in reducing the incidence of coronary heart disease, perhaps by altering properties plasma lipoproteins. The hypothesis that omega-3 polyunsaturated fatty acids (PUFA) produce changes lipid ordering and dynamics within high density lipoprotein (HDL), thereby potentially modifying cholesterol transport, is investigated here. Rabbits were fed a diet supplemented with either 10% (by weight) menhaden oil (MO), rich PUFAs, or hydrogenated cottonseed for period 12 weeks. HDL was isolated sequential flotation ultracentrifugation from drawn every 2 Gas chromatography confirmed predominant PUFAs oils, eicosapentaenoic 20:5 docosahexaenoic 22:6 acids, only incorporated into triglyceride, phospholipid, cholesteryl ester constituents lipoproteins rabbits on MO diet. ESR 5- 16-doxyl stearic demonstrates molecular order outer monolayer virtually unaffected. In contrast, 12-doxyl stearate indicates less inner apolar core than Fluorescence polarization 1,6-diphenyl-1,3,5-hexatriene supports this finding. greater disorder detected may result an enhancement exchange between cells, which have anti-atherogenic ramifications.