Small molecular ion adsorption on proteins and DNAs revealed by separation techniques.

摘要: Ion binding is a term that assumes the ion included in solvation sphere characterising biomolecule. The forces are not clearly stated except for electrostatic attraction; weak (hydrogen bonds and Van der Waals forces) likely involved. Many publications have dealt with to proteins consequences over past 10 years, but only few studies were performed using high-performance liquid chromatography (HPLC: exchange, reversed phase without well-identified immobilised metal affinity chromatography) capillary zone electrophoresis (CZE). This review focuses on of DNAs mainly oxyanions (phosphate, borate, citrate) amines used as buffers both HPLC eluent background electrolyte CZE. Such specific adsorption biomolecules evidenced by physico-chemical characteristics such mobility or retention volume, closely associated net charge, which differ from expected experimental data obtained under conditions an indifferent electrolyte. It shown key parameter repulsion between free protein fouled membrane ultrafiltration separation mixture.