VP1, the Putative RNA-Dependent RNA Polymerase of Infectious Bursal Disease Virus, Forms Complexes with the Capsid Protein VP3, Leading to Efficient Encapsidation into Virus-Like Particles

摘要: A cDNA corresponding to the coding region of VP1, putative RNA-dependent RNA polymerase, infectious bursal disease virus (IBDV) was cloned and inserted into genome a vaccinia inducible expression vector. The molecular mass antigenic reactivity VP1 expressed in mammalian cells are identical those its counterpart IBDV-infected cells. results presented here demonstrate that is efficiently incorporated IBDV virus-like particles (VLPs) produced coexpressing polyprotein VP1. Incorporation VLPs requires neither presence RNAs nor nonstructural polypeptide VP5. Immunofluorescence, confocal laser scanning microscopy, immunoprecipitation analyses conclusively showed forms complexes with structural VP3. Formation VP1-VP3 likely be key step for morphogenesis particles.